Content I Started

find . -type f -exec grep -l "In order to continue, you need to verify that you're not a robot by solving a CAPTCHA puzzle" {} \; | wc -l 2310 Ugh add -exec rm {} + to delete (WITHOUT the wc -l) I'm going to try to rerun now, there's been some time passed since I last did it.

bitter supposedly stimulates GLP-1 through gut receptors, maybe this is also why I seem to become GLP-1 resistant..

44 years old...

I'll have to buy a suitable dynamometer. Only issue with this kind of measurements is that, it is not a constant, with training you can improve your grip strength substantially. Does this improve your mortality HR in a few weeks? And what if you have to discontinue training? Does it worsen your mortality HR in a short time?

https://github.com/mendableai/firecrawl https://forum.cloudron.io/topic/12140/firecrawl-on-cloudron-turn-any-site-into-llm-data-by-web-scraping/3 Claude Sonnet 3.5.1 has VERY interesting code I should try.

I started to post in that forum as well, it's more oriented to pharmacological interventions for longevity and the detail in this regard is extreme, the latest research is published and discussed with all minutiae.

https://www.sciencedirect.com/science/article/pii/S0304389421031216 Cotton and wood are biopersistent IN HUMAN TISSUE too. It's this kind of study that makes me panic about microplastics less, even *after* that infamous 2024 "microplastics in brains" study [it seems that this year is confirming our worst fears about MPs **and worse**], but it's still possible that microplastics may be unusually likely to lodge in the brain relative to wood/cotton - I don't know. Wood cellulose fibers persist in rat lungs after 1 year, higher bio-persistence compared to asbestos. Estimated half-life about 3 years (Muhle et al., 1997)

Strawberries, prunes, dates, quaker lightly salted rice cakes.

I went an oxford instruments event today! Edinburgh instruments has a v. good channel https://scix2024.eventscribe.net/SearchByExpoCompany.asp?pfp=BrowseByCompany there are also mit.nano events

the 6th is most informative. uV values are important for many reasons (they're important for tracking brain health) [there are also some separate ICA images too] CHAL_NG_1.bmp CHAL_NG_2.bmp CHAL_NG_3.bmp CHAL_NG_4.bmp CHAL_NG_5.bmp CHAL_NG_6.bmp CHAL_NG_7.bmp CHAL_NG_8.bmp CHAL_NG_9.bmp CHAL_NG_10.bmp CHAL_NG_11.bmp CHAL_NG_12.bmp CHAL_NG_13.bmp

Cool Alex! How'd you get the forum back up?

There are new DNAm aging clocks that are better at capturing this effect now..

It never even went down until I ate Amy's tortilla soup and then it went below 100.. A lot of Amy soup produce low response, they are lower calorie density than garbanzo beans But kidney beans also lower response

Age-dependent changes in the kidney include a decrease in the relative abundance of mesangial cells, capillary endothelial cells, loop of Henle ascending limb epithelial cells and loop of Henle thick ascending limb epithelial cells (Figure 2g). Both mesangial cells and capillary endothelial cells are core glomerular cells and their relative abundances reduction (Figure 2g top panels), together with a tissue-wide reduction of Egf and Atp1a1 expression (Figure 2h; Supplementary Table 6) suggest impaired glomerular filtration rate29,30. Interestingly, local Atp1a1 expression actually increases with age in both capillary endothelial cells and mesangial cells, suggesting that a compensation mechanism counteracts the effects of the cell proportion declining with age. This finding is reinforced by differential gene expression results indicating that uromodulin (Umod), the most abundant protein in urine31, is also reduced in expression across the tissue. Umod is produced by the epithelial cells that line the thick ascending limb, and therefore given the relative decrease in the proportion of epithelial cells in the ascending and thick ascending limb, our results suggest that normal kidney functions are impaired32 (Figure 2g bottom panels, Figure 2h; Supplementary Table 6). As with Atp1a1, we see that Umod expression increases in a cell type whose abundance decreases with age, leading to an overall reduction of Umod expression in the organ. In spleen the proportion of T cells decreases with age while the relative amount of plasma cells increases (Figure 2i). This is supported by upregulation of B/plasma cell markers (Cd79a, Igj; Figure 2j; Supplementary Table 6) and downregulation of Cd3d (Figure 2j; Supplementary Table 6). Similarly, in mammary gland we observed a decline of the T cell population (Extended Data Figure 5c). Age-related decline of T cell populations has been associated with increased risk of infectious disease and cancer33 and our results suggest this may also happen in spleen and mammary gland. Moreover, AP1 transcription factors34 (Junb, Jund and Fos) were upregulated with age (Extended Data Figure 5d; Supplementary Table 6), consistent with the observation that normal involution of the mammary gland is accompanied by increased expression of this gene family35. The cellular composition of each tissue tends to vary with age, and we investigated changes for tissues with at least three time points (Supplementary Table 5). Since dissociation does not affect all cell types in a tissue equally, changes in the relative composition of a given cell type with age are more meaningful than comparing proportions of different cell types at a single age22–24. The bladder has pronounced changes in cell type composition with age (Figure 2e). While the mesenchymal compartment of this tissue decreases by a factor of three over the lifetime of the mouse (Figure 2e left), the urothelial compartment increases by a similar amount (Figure 2e right). The observation that the bladder urothelial cells increase with age is concordant with known age-related urothelial changes25. Differential gene expression (DGE) analysis of overall tissue changes with age revealed that stromal-associated genes (Col1a1, Col1a2, Col3a1, Dcn) are downregulated while epithelial-associated genes (Krt15, Krt18, Sfn) are upregulated, supporting the compositional observations (Figure 2f; Supplementary Table 6). The decline of the endothelial population suggests that bladder aging in mice may be associated with lower organ vascularization, consistent with recent findings26,27 and with the observed downregulation of vasculature associated genes Htra1 and Fos (Figure 2f; Supplementary Table 6). The increase in the leukocyte population could indicate an inflammatory tissue microenvironment, a common hallmark of aging which is consistent with literature on overactive bladders28 and supported by a significant overexpression of Lgals3, Igfbp2 and Ly6d across the tissue (Figure 2f; Supplementary Table 6) and by the overexpression of immune response associate genes such as Tnfrsf12a and Cdkn1a, by both bladder (mesenchymal) cells and bladder urothelial cells (Supplementary Table 6). Moreover, when comparing across ages, we observed that old leukocytes show increased expression of pro-inflammatory markers, such as Cd14, Lgals3 and Tnfrsf12a, and decreased expression of anti-inflammatory ones, such as Cd9 and Cd81 (Supplementary Table 6).

https://onlinelibrary.wiley.com/doi/10.1111/acel.14087

https://x.com/AlainGoriely/status/1790285747657822269 There's evidence for both directions, most people are just lazy so the averages are not easy to trust

Hate to feed Alex's paranoia but... p.s. I'm also interested in the unnamed series mentioned above, but the Alex 🤖 seems to have difficulty interacting with others 😭

https://www.rapamycin.news/t/syringic-acid-walnuts-olives-and-anti-neuronage/14269 Food products [mg/100 g] Thyme 11.70 ± 0.42 [49] Oregano 3.75 ± 5.30 [49] Sage 3.35 ± 4.74 [49] Rosemary 1.03 ± 1.79 [50] Cloves 0.79 ± 0.00 [51] Walnut 33.83 ± 13.96 [52] Black olive 33.10 ± 32.13 [53] Green olive 6.00 ± 8.49 [52] Cauliflower 1.13 ± 0.02 [52] Date (dried) 6.06 ± 3.81 [54] Date (fresh) 2.45 ± 4.10 [54] Currant 0.34 ± 0.13 [55] Grape seed (Cabernet Sauvignon) 122.87 ± 0.25 [56] Pumpkin pulp (C. maxima ‘Bambino’) 2.67 ± 0.05 [57]

PFAS is often in tea bags (this is why I remove the bag before pouring)... Tejava uses giant tea bags, I might have to externally test it b/c I'm starting to drink a lot of Tejava (testing water is $70). I'm not as concerned about PFAS as I am with microplastics, and I don't do much takeout, but amounts in tea could be concerning.

https://www.nature.com/articles/s41514-024-00150-8 more pro-antioxidants

I'm on CR; I don't need or want semiglutide. -- Saul

https://www.linkedin.com/pulse/provide-notebooklm-your-papers-get-down-key-few-ken-wasserman-l0xte/?trackingId=QR6U%2BMm3qwrJBQMUHzHoKA%3D%3D

https://www.dsquintana.blog/how-to-perform-a-bayesian-meta-analysis-in-r/ https://archive.ph/Vqyaw