General Health and Longevity Latest Topics

What is your grip strength?

Thu, 31 Oct 2024 15:38:52 +0000

68lb right/64 lb left.
was 30kg in late 2021 [practically the same]

I’m in between males and females [I may have low testosterone/DHT signalling which would make sense]

on 2020-11-29, it was 25.5 kg, 58-60lbs at max

God I had measurements earlier I can’t find.

Fasting (Without Calorie Restriction) And Calorie Restriction With Fasting Extend Lifespan

Sun, 27 Oct 2024 11:09:52 +0000

Drinking/tap water thread (and ideal water filters/pitchers)

Thu, 11 Jan 2024 22:04:27 +0000

Quote

Huberman lists some but he doesn't even capture all of the issues

https://www.pnas.org/doi/10.1073/pnas.2300582121

Water filtration creates its own MP contamination.. if you look at the graph, THE MAJORITY OF NPS are not from the bottle, but from the water itself...

Ideally, you'd test RIGHT BEFORE filtration and right after filtration

https://www.linkedin.com/feed/update/urn:li:activity:7150600847725383680/

The 2024 recent microplastics papers (above) also show that contamination comes from the REVERSE OSMOSIS FILTER ITSELF (RO filters are made of plastic too), so b/c of this I'll still rely on my pitcher

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some testing services [non-ideal]

https://mytapscore.com/products/advanced-city-water-test

THEY STILL ALL LEAVE OUT THE MOST IMPORTANT: POLYSTYRENE AND PVC

https://mytapscore.com/products/microplastics-water-test?_pos=1&_sid=6135ed9de&_ss=r

is microplastics but i dont know what a report looks like.. you know, you could test the water at universities where the water is supposed to be super-clean... [and where a case can be argued for especially high social importance..]

====

Quote

Do you do water testing of canned beverages? Also do you do nanoplastics testing? What does a microplastics testing report look like? (does it have separate scores for polystyrene?)

Hi again

When you say "canned beverages" are you referring to something like bottled water? Or a non-water product (like a milk or juice)?

We currently do not offer any nanoparticle testing - as there are no commercially avaialbe methods for nanoparticles and nanoplastics (its typically only avaialbe in speciality university and academic reserach labs)

Our microplastics tests does not specifically identify the material of the plastics found. It only does concentration of microplastics (by particle size) and typically the plastic particles assoicated with bottled water and other packaged beverages are so small that are not very well captured by that test - so I just want to be transparent that it may not be a good fit

based on what you are describing/looking for

🙂

Is carnosine worth it?

Sat, 01 Jun 2024 12:24:58 +0000

I was using carnosine for several years since I read an article showing 20% lifespan extension in mice fed with carnosine. There were also several human studies showing decrease in AGE formation but I think all of them used obese/diabetic individuals. As the supplement is quite expensive (~40$ per month) I am considering if it is worth such money so recently I revised the available studies.
Turned out that the most promising study, the one that showed life extension in mice was using senescence accelerated mice and it improved only average not maximal lifespan:
http://protein.bio.msu.ru/biokhimiya/contents/v65/full/65071018.html
Strangely , I couldn't find any more lifespan studies with this supplement, the one above was done over 20 years ago. Moreover for some reason SENS foundation members never mention it and decided to go with other agents in their anti AGE program. Seems strange that nobody in anti ageing field further tried to explore it and it remained pretty obscure comparing with other supplements like NAD or astaxanthin. Is there some reason for that I am missing? It seems like a good candidate for ITP study.
Have you heard about more recent studies using carnosine?

How Not To Age: Dr. Michael Greger

Sat, 09 Sep 2023 01:06:09 +0000

Dr. Greger's video trailer for his new book was released today. I personally am beyond excited for it and have pre-ordered my hardcover copy from Amazon here. The book is currently ranked #2 of all Amazon books in the Diets and Weight-loss category.

Uncover the evidence-based science to slowing the effects of aging, from the New York Times bestselling author of the How Not to Die series

When Dr. Michael Greger, founder of NutritionFacts.org, dove into the top peer-reviewed anti-aging medical research, he realized that diet could regulate every one of the most promising strategies for combating the effects of aging. We don’t need Big Pharma to keep us feeling young―we already have the tools. In How Not to Age, the internationally renowned physician and nutritionist breaks down the science of aging and chronic illness and explains how to help avoid the diseases most commonly encountered in our journeys through life.

Physicians have long treated aging as a malady, but getting older does not have to mean getting sicker. There are eleven pathways for aging in our bodies’ cells and we can disrupt each of them. Processes like autophagy, the upcycling of unusable junk, can be boosted with spermidine, a compound found in tempeh, mushrooms, and wheat germ. Senescent “zombie” cells that spew inflammation and are linked to many age-related diseases may be cleared in part with quercetin-rich foods like onions, apples, and kale. And we can combat effects of aging without breaking the bank. Why spend a small fortune on vitamin C and nicotinamide facial serums when you can make your own for up to 2,000 times cheaper?

Inspired by the dietary and lifestyle patterns of centenarians and residents of “blue zone” regions where people live the longest, Dr. Greger presents simple, accessible, and evidence-based methods to preserve the body functions that keep you feeling youthful, both physically and mentally. Brimming with expertise and actionable takeaways, How Not to Age lays out practical strategies for achieving ultimate longevity

Healthy Tea Recommendations?

Tue, 20 Sep 2022 00:06:10 +0000

I adore coffee. It's delicious, healthy, it keeps me motivated, and distracts the great dragging black dogs of depression.

But I got carried away. Now it's turned against me. Coffee makes me happy for hours, and then horribly anxious for even more hours. So after a long and sad decontamination journey I've finally ramped off. I truly miss it, though, and believe in coffee's magical powers, so I know I'll return back to it again someday.

Meanwhile, I'm transitioning back again to teas and wondered what world's healthiest brands you're drinking nowadays? White tea, green, hibiscus, what do you like, and where do you buy it?

195,500 particles of microplastic per gram of apple? 126,150 particles per gram of broccoli?

Mon, 02 Jan 2023 01:23:36 +0000

https://www.sciencedirect.com/science/article/pii/S0013935120305703

https://www.dailymail.co.uk/sciencetech/article-8459727/Microplastics-contaminating-fruit-vegetables-eat-including-apples-lettuces.html

I fainted when I read this… If there’s any source of consolation, how many particles of air pollution do we breathe in per day? (given an average µg/m³ of 10) And are the numbers *worsening* over time (this is the big uncertainty)

posting more thoughts here -https://forum.longevitybase.org/t/how-to-reduce-microplastics/126 and https://www.rapamycin.news/t/195-500-particles-of-microplastics-per-gram-of-apple-126-150-particles-per-gram-of-broccoli/4734/10

Metformin - yet another u-turn?

Mon, 18 Sep 2023 09:38:11 +0000

Metformin's claimed effects in healthy (i.e. non-diabetic) humans have certainly been full of twists, turns and reversals. Extends life, cuts down on cancer; no it doesn't and not in non-diabetics. Blunts or eliminates the benefits of exercise, poor effects on vo2max and muscle training.

Latest plot twist: attenuates muscle atrophy (in the immobilized), generally slows the senescence of muscle tissues and keeps those cells young in older people:

https://onlinelibrary.wiley.com/doi/10.1111/acel.13936

Stay tuned, I'm sure another 180 is just around the corner.

Is C15 A Good Measure Of Aging?

Sun, 20 Oct 2024 11:12:57 +0000

https://www.youtube.com/watch?v=iQHugpi8b7k

[IMPORTANT] Favorite low-effort, lower-calorie food items that you can easily get from a store/use as fast food. #DiningGuide

Thu, 29 Feb 2024 23:41:28 +0000

https://www.rapamycin.news/t/what-are-your-favorite-low-er-calorie-low-calorie-per-volume-ratio-or-appetite-suppressing-foods/3625/8 (but I need to repost them here b/c this forum is friendlier to long content, and there are trashy replies)

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(I know many of these foods have high sodium content, so add potassium salt to neutralize). Alignment/human power/all other outcomes are instrumentally convergent on making everyone's diets lower-effort *and* healthier, and I've optimized for both 🙂

Sodastream water maker for carbonating water (linked version is glass). Carbonated water is way more filling and often fills me up enough when I get cravings. Prefilter water with a decent water filter (maybe SimplyFiltered) to reduce PFAS and other contaminants (and maybe microplastics)
almond/macademia milks. They are SO filling. they just make you pee a lot. Make sure to get low-calcium versions… They are often packed in Tetrapak plastic (whose effects/leachability relative to the terrible plastics in bottled water are currently unknown but socially important to investigate - pls ping https://www.publichealth.columbia.edu/news/bottled-water-can-contain-hundreds-thousands-nanoplastics). Soy milk exists too, but has more protein than almond/macademia milk (and is 80 calories/serving [640 in one carton], unlike almond milk's 40 calories/serving)
pickled vegetables. VERY tasty and can substitute for other cravings. Just beware of the emulsifier polysorbate 80. Has vinegar. The processing can reduce pesticide load. Also, pickled cucumbers can irritate the stomach lining - Podcast: What Is H. Pylori? - YouTube 2. There's an amazon form in glass jars (a bit more expensive)
coffee (preferably ground green) in soy/almond/macademia milk prepared via all-steel coffee percolator. Excellent appetite suppressant (along with green tea).
Tomatoes and cucumbers (easy to snack on, low bitterness). Tomatoes are really a fruit with a calorie density lower than many vegetables (even cruciferous ones). Greenhouse/Canadian tomatoes have much lower pesticide than Mexican tomatoes. Cherry tomatoes are way cheaper in Europe
Parsley (one of only two veggies to decrease Mike Lustgarten's rate of epigenetic aging). Lots of apigenin
giardiniera (glass jars available for shipping over amazon)
ezekiel bread (flour-free and low-GI - one of the *few* healthy breads. Currently testing on my CGM). 11-14% protein and 8% fiber by weight, which is higher than most beans. Boiled black beans are 5-7% protein *and* fiber by weight, chickpeas are ~8% protein/fiber by weight
Nopalitos tender cactus (from La Costena) => in glass containers too! Found in the mission. My only concern is that they’re imported from mexico, and growing standards in mexico are not as good (aka there’s higher chance of contamination, though one would hope that since they’re cacti, they may be grown farther from microplastic/pesticide-contamined areas - though I’m less sure about pesticides b/c Mexico produce has higher contamination)’
Miso soup (often cheapest/only affordable option at restaurants too)
carrots (super-cheap). Can destroy your teeth if you eat too many raw (and this is an issue when you already eat enough acidic food)
Salsa. Best vegetable dip ever (tho add potassium to neutralize the Na). Or I just drink out of the jar. Has vinegar. Black bean salsa can help satiate cravings that salsa alone cannot. Enough salsa can make raw vegetables more palatable. Easy to mass-order off Amazon
Seaweed of all forms (Thiel Fellow Reiss Jones has a Seaweed Gang whatsapp groupchat just for bulk purchases of them). Brown (non-tropical) seaweed can serve as SIRT6 activators. More expensive than veggies, but could change (and even despite the expense, they're powerful in small amounts)
mustard (very low calorie flavor), also very juicy and substitutes for a lot of other cravings. Dijon is better. You can get dijon shipped via amazon though it has added sugar (idk how much)
Strawberries. Calorie-density of a vegetable [151cals/lb], tho they do spike glucose (try to coadminister with 300mg canagliflozin). Very pesticide-heavy if non-organic (target has affordable organic).
Strawberry leaves and blueberry leaves (yes, they are edible and healthier than the fruit 🙂 )
wild apples (more bitter, before they got bred to be sweet). Also all the fruit before they got mass-bred to be sweet
ALL the vegetables (this is obvious and goes without saying, though many are hard to snack on as fast-food, and the value of time only increases as AGI nears - hence why I included everything else). Broccoli, cucumbers, tomatoes => easiest to use as fast food. Everything is easier in an instant pot (or you can put a bunch of water over them and boil them => you’ll hear it when the water boils/pours over and then they’re ready). I prefer electric stoves over gas stoves b/c safer. Most cruciferous vegetables have lower pesticide loads.
Watercress Is The Healthiest Vegetable? Carrot is #26? Here’s A Chart To... 11
https://optimisingnutrition.com/too-much-nutrients/#more-36003 5 | What Influences Satiety: Carbs, Fat, and More | Optimising Nutrition 1 | https://public.tableau.com/app/profile/marty.kendall7139/viz/satietyindex-forecastdailyintakevsdietqualityscore/topdownwithoutcholesterol 2 |
VEGETABLE SOUP (too bad they’re hard to buy in stores, and costs can easily blow up with volume). Costs don’t blow up with volume in Ivy League dining halls
in university dining halls that are all-you-can-eat [all the Ivies, JHU, UMass-Amherst, UMichigan, etc], it’s super-easy to get an abundance of soup and low-calorie vegetables (sauteed in olive oil, though not many are boiled)
if you’re friends with some people in CR Society (or a certain Jason A), you can sometimes enjoy their super-specially prepared vegetables (too bad I don’t have the time to prepare them)
anything with vinegar (enhances glycemic control, helps one fill full)
keto bread (esp unbun). A lot of them have lots of psyllium husk/insoluble fiber. Whole foods has nobagels. ThinSlim foods has some. They have egg protein (which I don't consider a concern given how filling they are). It's unclear if unbun is healthier than bread/beans + acarbose/canagliflozin (which can completely neutralize the glycemic spike). Bread increases Steve Horvath's GrimAge... (I once gave him acarbose tho 🙂 )
Jellyfish (their amino acids are mostly collagen) and they have low calorie-density. Also, they don't have brains and cannot suffer. If you pay close attention, jellyfish and unbun keto bread are the only two non-vegan foods on the list
Shirataki noodles (they would be a miracle if they weren't always packaged in the most sus of plastic containers)
things sweetened with sugar alcohols (tho really, who needs sweeteners?)
spices: https://www.mdpi.com/2076-3921/8/4/100/htm - allspice, cloves, anise, chinese 5-spice have both anti-glycative and anti-ROS abilities.

Lower-calorie substitutes for other food (or just super-healthy/high-fiber food)

Peaches have low calorie density at 177 cals/lb (+you can use acarbose on). But often have pesticides.
Silken tofu has 45 cal/85 grams (much less than regular tofu for some reason)
Slimcado. Can substitute for fat/nut cravings
Chia seeds are 34% fiber by weight, flaxseeds are 29% fiber by weight. No other food is so high fiber by weight (though vegetables have a lot of non-fiber bulk)
POSSIBLY corn bran (lots of insoluble fiber) - they are sold in Honduras. Supposedly lower-calorie than wheat bran (only from one source). I felt knocked out after consuming them => not a good indication of “low-calorie” b/c I know when too many carbs knock me out. Eat with acarbose
beans/lentils => combine with acarbose and the blood sugar spike is super-low. Can be used as a primary source of calories. They can knock me out, however.
mangoes. More sucrose than any other fruit so you can blunt the glucose spike with acarbose (which is cheaper and more immediately applicable than SGLT2 inhibitors). Still they knock me out if I eat too many.
https://www.quora.com/Does-acarbose-work-for-reducing-the-glycemic-index-of-fruit (acarbose-safe fruit). NOTE: acarbose does not work on dragonfruit. Oranges spike my glucose way less than apples (despite lower fiber).

https://www.rapamycin.news/t/what-is-the-chemical-composition-and-kcal-g-of-the-polysaccharides-oligosaccharides-raffinoses-non-digestible-fraction-found-in-beans-vs-bread-rice-corn-pasta-and-other-starches/4301

Prickly pear is also 185 calories/lb which is less than pear calories (still higher than strawberry calories, similar to peach calories)

Olives are normally healthy but they often contain acrylamide due to how they're processed. I have noticed that pasta sauce AND pomi's crushed tomatoes spike my glucose (which could be the same for tomato sauce and salsa)

Previous guide:

https://forum.longevitybase.org/t/notes-on-nutrition-of-foods-esp-the-superfoods/293

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I sometimes get gourmet fermented vegetables from Cambridge Naturals (fermentation is good, esp fermenting natto). They're not really more expensive than restaurant food, so for all the money I save NOT going to restaurants, I can use it for these. They are still expensive enough that I can't regularly eat them (tho AGI timelines make this expense matter way less and if you invested enough crypto=>AMD/NVIDIA...)

https://cambridgenaturals.storebyweb.com/s/1000-4/b?g=MGP-1000-21&g=MGP-1000-89

tapenades are great dips for veggies (+ultra healthy+often come in glass containers). I ate tomatoes+mushrooms+onions+cauliflower today, and NONE of them did anything to make me feel full. I then dipped mushrooms in tapenade AT LAST and it did it.

Sea moss is a wonderful appetite suppressant

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Garden Vegetable Soup 1 (trader joe's)

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Shirataki/miracle noodles. They are a low-calorie miracle, only that they don’t really get digested (you don’t want to eat too much at once b/c indigestion) and they’re always packaged in the most suspicious sharp plastics ever (I’d buy them in abundance if not for the plastics issue - fortunately I guess I have enough vegetables to distract myself with).

THIS is the most common food I’ve been consuming over the past month (mostly because it’s very cheap - the cheapest to order off amazon):

[it is HELLA addicting when you add olive oil over it]. Plastics are a concern but amazon prime doesn’t allow for much else (and the plastics don’t seem that shardy like water-bottle plastics - like - not all plastics are equally “evil” and certain plastics just “feel” much worse than others [like pointier microplastics from waterbottles/shirataki seem more likely to insert themselves into cell membranes, and HDPE/PP plastics seem more “benign” than ones made of weirder materials - never mind that you want to avoid them if you still can]).

among breads, there is 7 base culture keto bread and ezekiel [tho ezekiel you should take with acarbose]

passionfruit is 11% fiber by weight (but 428 calories/lb)

Aubrey de Grey resurfaces, predicts 50% chance of longevity escape velocity within 15 years

Thu, 17 Oct 2024 23:45:38 +0000

Red Light Therapy Improves Mitochondria Function, Eye Health, And Lifespan: Glen Jeffery, PhD

Wed, 16 Oct 2024 19:54:37 +0000

QEEG report of brainwaves with raw uV values (got them from someone who fed my data into neuroguide)

Wed, 16 Oct 2024 19:40:33 +0000

the 6th is most informative.

uV values are important for many reasons (they're important for tracking brain health)

[there are also some separate ICA images too]

CHAL_NG_1.bmp CHAL_NG_2.bmp CHAL_NG_3.bmp CHAL_NG_4.bmp CHAL_NG_5.bmp CHAL_NG_6.bmp CHAL_NG_7.bmp CHAL_NG_8.bmp CHAL_NG_9.bmp CHAL_NG_10.bmp CHAL_NG_11.bmp CHAL_NG_12.bmp CHAL_NG_13.bmp

What are the most important genome/SNP/SNPedia sites to query from a VCF file of one's genome? How do I visualize my genomic data

Wed, 04 Oct 2023 21:41:38 +0000

https://knausb.github.io/vcfR_documentation/visualization_2.html

These aren't the **most** critical, but there are some variants for plasmalogens, some variants for how well one digests kimchi, COMT variants, etc..

There are some FOXO variants:

https://www.rapamycin.news/t/just-dna-seq-open-source-longevity-genetics-generator-see-your-oakvar-report-of-your-genomic-longevity-variants/4481/3 (this is so preliminary it's hard to interpret, there is no way in hell my PRS risk score is 92%).

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for nebula sequencing they use this as reference file: ##reference=file:///mnt/ssd/MegaBOLT_scheduler/reference/hg38.fa

here are some example variants on maybe more actionable things (like methylation):

Quote

I am taking Prodrome Glia for white matter inflammation issues derived from Lyme Disease. I haven’t found a single thread discussing Dayans work other than this. So please keep the discussion going.

I suspect my issue is that I have several genetic variants that might be contributing:

rs7946 T/T:

PEMT pathway is responsible for the body’s production of phosphatidylcholine, which is part of the phospholipid bilayer making up the membranes surrounding our cells. The PEMT enzyme is key in the body’s ability to create choline. Genetic variants that decrease the function of the enzyme cause greater reliance on choline from dietary sources.

rs10791957:
> - A/C: reduced turnover of methionine to phosphatidylcholine

Plus:

rs18J01133: >- A/G: one copy of **MTHFR C677T** allele, enzyme function decreased by 40%

Haven’t tested my father on prodrome (yet), but for me, I tested on the 2nd percentile for Total PhosphatidylCholine at age 32 and with a healthy diet. After 3 months of taking 10g a day of Sunflower lecithin I only went up of the 12th percentile. So I am taking now PC capsules in addition. Will test again soon and share my results.

my MTHFR variants (only for regulatory variants, this does not contain 1298 A>C (rs1801131)😞

Quote

chr1    11791061        rs7518348       A       G       656.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=25;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=28.56;SOR=1.270;VQSLOD=6.39;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,23:24:69:685,69,0
chr1    11791893        rs6700268       C       G       1397.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=53;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=26.37;SOR=1.654;VQSLOD=4.40;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,53:53:99:1426,156,0
chr1    11792912        rs4846050       T       C       955.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=35;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=29.87;SOR=1.179;VQSLOD=7.11;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,32:33:96:984,96,0
chr1    11793149        rs5772477       C       CT      291.74 PASS    AC=2;AF=1.00;AN=2;DB;DP=14;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;QD=29.17;SOR=1.085;VQSLOD=3.91;culprit=FS GT:AD:DP:GQ:PL 1/1:0,10:10:30:329,30,0
chr1    11793450        rs542060620     CTTTTTTTTTTTT   C       887.73 PASS    AC=2;AF=1.00;AN=2;DB;DP=23;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;QD=27.39;SOR=1.828;VQSLOD=3.89;culprit=FS GT:AD:DP:GQ:PL 1/1:0,21:21:63:925,63,0
chr1    11793542        rs7535669       G       C       997.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=32;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=33.26;SOR=0.976;VQSLOD=7.44;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,30:30:89:1026,89,0
chr1    11794400        rs4846051       G       A       1567.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=43;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=32.14;SOR=1.005;VQSLOD=7.28;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,42:42:99:1596,126,0
chr1    11794698        rs1994798       G       A       1393.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=38;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=35.10;SOR=0.693;VQSLOD=7.19;culprit=QD      GT:AD:DP:GQ:PL 1/1:0,38:38:99:1422,114,0
chr1    11795810        rs6541003       G       A       1436.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=37;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=32.12;SOR=1.061;VQSLOD=7.28;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,36:36:99:1465,108,0
chr1    11796321        rs1801133       G       A       561.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=0.712;DB;DP=39;ExcessHet=3.0103;FS=1.243;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=14.40;ReadPosRankSum=-1.307e+00;SOR=1.022;VQSLOD=4.03;culprit=FS   GT:AD:DP:GQ:PL 0/1:20,19:39:99:590,0,616
chr1    11796764        rs7545331       G       C       1705.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=51;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=34.12;SOR=0.952;VQSLOD=6.73;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,50:50:99:1734,149,0
chr1    11797183        rs7526128       C       T       668.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=21;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=32.00;SOR=1.402;VQSLOD=5.01;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,19:20:57:697,57,0
chr1    11797196        rs1323763550    CAA     C       555.73 PASS    AC=2;AF=1.00;AN=2;DB;DP=17;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;QD=36.56;SOR=1.112;VQSLOD=3.54;culprit=QD GT:AD:DP:GQ:PL 1/1:0,14:15:42:593,42,0
chr1    11797324        rs6541004       T       C       1073.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=34;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=32.54;SOR=1.542;VQSLOD=5.60;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,33:33:98:1102,98,0
chr1    11797353        rs7537955       A       G       1384.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=40;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=27.18;SOR=2.133;VQSLOD=5.81;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,39:39:99:1413,117,0
chr1    11797468        rs6541005       A       T       1629.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=45;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=35.20;SOR=0.798;VQSLOD=6.53;culprit=QD      GT:AD:DP:GQ:PL 1/1:0,37:38:99:1658,111,0
chr1    11797469        rs6541006       A       T       1771.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=45;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;QD=28.16;SOR=0.798;VQSLOD=4.88;culprit=FS GT:AD:DP:GQ:PL 1/1:0,37:38:99:1800,120,0
chr1    11797894        rs4846052       T       C       1172.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=43;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=27.92;SOR=0.788;VQSLOD=6.44;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,42:42:99:1201,124,0
chr1    11798615        rs4845885       T       C       1176.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=45;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=27.37;SOR=1.312;VQSLOD=5.57;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,43:43:99:1205,128,0
chr1    11799852        rs7527897       A       G       951.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=33;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=29.74;SOR=0.957;VQSLOD=6.64;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,32:32:96:980,96,0
chr1    11800063        rs11121832      T       C       946.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=35;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=27.05;SOR=1.002;VQSLOD=6.57;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,35:35:99:975,104,0
chr1    11800626        rs7533315       T       C       1065.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=37;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=29.60;SOR=1.061;VQSLOD=6.77;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,36:36:99:1094,108,0
chr1    11801923        rs200892006     CAAAAAAAAAAAAA C       1043.73 PASS    AC=2;AF=1.00;AN=2;DB;DP=29;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;QD=28.11;SOR=1.051;VQSLOD=5.57;culprit=FS GT:AD:DP:GQ:PL 1/1:0,24:24:73:1081,73,0
chr1    11802157        rs9651118       T       C       566.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=-2.869e+00;DB;DP=38;ExcessHet=3.0103;FS=0.000;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=15.74;ReadPosRankSum=-1.284e+00;SOR=0.703;VQSLOD=5.44;culprit=FS      GT:AD:DP:GQ:PL 0/1:17,19:36:99:595,0,585
chr10   11791026        rs6602533       T       C       1114.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=38;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=30.97;SOR=1.209;VQSLOD=6.78;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,36:36:99:1143,107,0
chr10   11792003        rs35420609      C       A       581.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=2.23;DB;DP=43;ExcessHet=3.0103;FS=4.506;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=13.85;ReadPosRankSum=0.933;SOR=0.954;VQSLOD=1.78;culprit=ReadPosRankSum     GT:AD:DP:GQ:PL 0/1:22,20:42:99:610,0,674
chr10   11792352        rs17554109      G       A       430.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=3.28;DB;DP=30;ExcessHet=3.0103;FS=8.557;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=15.95;ReadPosRankSum=-1.274e+00;SOR=1.000;VQSLOD=2.19;culprit=FS    GT:AD:DP:GQ:PL 0/1:13,14:29:99:459,0,294
chr10   11792872        rs764165007     TGAG    T       499.73 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=1.30;DB;DP=33;ExcessHet=3.0103;FS=8.139;MLEAC=1;MLEAF=0.500;MQRankSum=-1.320e+00;QD=16.12;ReadPosRankSum=0.414;SOR=0.120;VQSLOD=0.797;culprit=FS      GT:AD:DP:GQ:PGT:PID:PL:PS       0|1:17,14:31:99:0|1:11792872_TGAG_T:537,0,672:11792872
chr10   11792877        rs75620717      G       T       508.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=2.17;DB;DP=31;ExcessHet=3.0103;FS=8.139;MLEAC=1;MLEAF=0.500;MQRankSum=-1.320e+00;QD=16.41;ReadPosRankSum=1.01;SOR=0.120;VQSLOD=0.790;culprit=SOR      GT:AD:DP:GQ:PGT:PID:PL:PS       0|1:17,14:31:99:0|1:11792872_TGAG_T:537,0,672:11792872
chr10   11792880        rs762076012     C       CCAG    571.73 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=-8.830e-01;DB;DP=33;ExcessHet=3.0103;FS=8.039;MLEAC=1;MLEAF=0.500;MQRankSum=-1.081e+00;QD=17.33;ReadPosRankSum=0.661;SOR=0.120;VQSLOD=0.780;culprit=FS        GT:AD:DP:GQ:PGT:PID:PL:PS       0|1:17,16:33:99:0|1:11792872_TGAG_T:609,0,666:11792872
chr10   11792882        rs78887114      C       T       580.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=2.11;DB;DP=34;ExcessHet=3.0103;FS=8.039;MLEAC=1;MLEAF=0.500;MQRankSum=-1.081e+00;QD=17.60;ReadPosRankSum=0.090;SOR=0.120;VQSLOD=1.42;culprit=SOR      GT:AD:DP:GQ:PGT:PID:PL:PS       0|1:17,16:33:99:0|1:11792872_TGAG_T:609,0,666:11792872
chr10   11792941        rs4750114       C       G       1327.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=38;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;QD=27.48;SOR=1.432;VQSLOD=4.50;culprit=FS GT:AD:DP:GQ:PL 1/1:0,37:37:99:1356,110,0
chr10   11792969        rs4750115       A       G       391.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=-3.952e+00;DB;DP=40;ExcessHet=3.0103;FS=4.649;MLEAC=1;MLEAF=0.500;MQRankSum=0.583;QD=10.05;ReadPosRankSum=-9.070e-01;SOR=1.473;VQSLOD=0.247;culprit=QD        GT:AD:DP:GQ:PL 0/1:22,17:39:99:420,0,770
chr10   11793513        rs7915245       C       T       1231.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=29;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=35.23;SOR=1.688;VQSLOD=4.77;culprit=QD      GT:AD:DP:GQ:PGT:PID:PL:PS 1|1:0,28:29:84:1|1:11793513_C_T:1260,84,0:11793513
chr10   11793515        rs7903322       T       C       1231.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=31;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;QD=31.95;SOR=1.688;VQSLOD=4.22;culprit=FS GT:AD:DP:GQ:PGT:PID:PL:PS       1|1:0,28:29:84:1|1:11793513_C_T:1260,84,0:11793513
chr10   11793661        rs201820594     TTTTTCTTTTCTTTTCTTTTCTTTTC      T       116.73 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=-1.282e+00;DB;DP=12;ExcessHet=3.0103;FS=0.000;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;QD=19.46;ReadPosRankSum=-1.834e+00;SOR=0.693;VQSLOD=2.26;culprit=FS GT:AD:DP:GQ:PL 0/1:2,4:6:71:154,0,71
chr10   11795657        rs7910181       C       T       187.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=0.275;DB;DP=10;ExcessHet=3.0103;FS=0.000;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;QD=18.78;ReadPosRankSum=-6.480e-01;SOR=0.569;VQSLOD=2.49;culprit=FS       GT:AD:DP:GQ:PL 0/1:3,7:10:84:216,0,84
chr10   11795825        rs4750119       G       A       1293.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=33;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=31.07;SOR=2.833;VQSLOD=5.59;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,33:33:99:1322,99,0
chr10   11796224        rs1310693610    C       CA      227.74 PASS    AC=2;AF=1.00;AN=2;DB;DP=11;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=25.30;SOR=0.892;VQSLOD=3.72;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,9:9:27:265,27,0
chr10   11796401        rs11257329      T       C       423.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=-4.224e+00;DB;DP=30;ExcessHet=3.0103;FS=3.890;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=14.61;ReadPosRankSum=-1.465e+00;SOR=0.198;VQSLOD=3.85;culprit=ReadPosRankSum GT:AD:DP:GQ:PL 0/1:12,17:29:99:452,0,392
chr10   11796670        rs6602534       T       C       982.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=32;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=30.71;SOR=0.818;VQSLOD=7.17;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,32:32:96:1011,96,0
chr10   11797031        rs10906019      T       A       218.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=-4.480e-01;DB;DP=30;ExcessHet=3.0103;FS=1.657;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=8.41;ReadPosRankSum=-6.690e-01;SOR=1.402;VQSLOD=1.62;culprit=QD       GT:AD:DP:GQ:PL 0/1:18,8:27:99:247,0,633
chr10   11797191        rs6602535       C       T       1183.77 PASS    AC=2;AF=1.00;AN=2;BaseQRankSum=1.76;DB;DP=33;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=29.68;ReadPosRankSum=0.00;SOR=0.307;VQSLOD=6.52;culprit=FS    GT:AD:DP:GQ:PL 1/1:1,31:32:86:1212,86,0
chr10   11798499        rs7908067       A       C       747.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=29;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=29.91;SOR=1.022;VQSLOD=6.50;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,25:26:73:776,73,0
chr10   11800133        rs3889052       C       A       920.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=24;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=36.61;SOR=1.519;VQSLOD=5.14;culprit=QD      GT:AD:DP:GQ:PL 1/1:0,24:24:72:949,72,0
chr10   11800226        rs1424133015    CT      C       102.90 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=1.38;DB;DP=8;ExcessHet=3.0103;FS=0.000;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=17.15;ReadPosRankSum=-6.740e-01;SOR=0.368;VQSLOD=3.75;culprit=FS     GT:AD:DP:GQ:PL 0/1:1,5:6:14:140,0,14
chr10   11800290        rs4356136       G       T       302.78 PASS    AC=2;AF=1.00;AN=2;DB;DP=8;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=29.67;SOR=1.179;VQSLOD=3.89;culprit=FS       GT:AD:DP:GQ:PL 1/1:0,8:8:24:331,24,0
chr10   11800954        rs1815306       C       T       1171.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=35;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=27.50;SOR=0.941;VQSLOD=6.67;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,33:34:98:1200,98,0
chr10   11801052        rs942530        C       T       1400.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=37;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=29.12;SOR=0.746;VQSLOD=7.10;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,36:37:99:1429,108,0
chr10   11801410        rs201612497     CTTTTTTTTT      C       1775.73 PASS    AC=2;AF=1.00;AN=2;DB;DP=43;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;QD=30.66;SOR=1.005;VQSLOD=5.44;culprit=FS GT:AD:DP:GQ:PL 1/1:0,40:42:99:1813,126,0
chr10   11803011        rs6602537       T       C       998.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=32;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=31.21;SOR=0.818;VQSLOD=6.83;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,32:32:95:1027,95,0
chr10   11803059        rs6602538       T       C       1105.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=37;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=29.89;SOR=0.746;VQSLOD=6.33;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,37:37:99:1134,110,0
chr11   11791074        rs72854808      A       T       862.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=26;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=28.49;SOR=0.963;VQSLOD=6.22;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,23:23:75:891,75,0
chr11   11791516        rs7940345       T       C       855.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=30;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=28.53;SOR=0.826;VQSLOD=6.52;culprit=QD      GT:AD:DP:GQ:PL 1/1:0,30:30:90:884,90,0
chr11   11791797        rs7950577       G       A       1563.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=45;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=36.69;SOR=0.990;VQSLOD=6.58;culprit=QD      GT:AD:DP:GQ:PL 1/1:0,44:44:99:1592,131,0
chr11   11792346        rs11022038      A       T       1400.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=38;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=25.02;SOR=1.179;VQSLOD=5.66;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,38:38:99:1429,114,0
chr11   11792467        rs376301547     T       C       385.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=-2.848e+00;DB;DP=28;ExcessHet=3.0103;FS=0.000;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;QD=15.43;ReadPosRankSum=1.38;SOR=0.839;VQSLOD=3.87;culprit=FS        GT:AD:DP:GQ:PL 0/1:9,16:26:99:414,0,266
chr11   11792629        rs16910148      C       T       1429.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=38;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=29.45;SOR=0.693;VQSLOD=7.19;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,36:36:99:1458,108,0
chr11   11793154        rs16910152      T       G       1119.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=36;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=31.10;SOR=1.371;VQSLOD=6.13;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,36:36:99:1148,107,0
chr11   11793613        rs150690263     AAAATT A       1782.73 PASS    AC=2;AF=1.00;AN=2;DB;DP=48;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=29.73;SOR=1.179;VQSLOD=6.51;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,41:43:99:1820,124,0
chr11   11794746        rs10831674      A       T       1182.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=35;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=28.95;SOR=0.693;VQSLOD=6.52;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,32:34:96:1211,96,0
chr11   11796517        rs16910159      G       A       625.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=19;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=29.04;SOR=0.804;VQSLOD=5.26;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,17:17:51:654,51,0
chr11   11797187        rs113190524     C       T       1743.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=50;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;QD=27.56;SOR=1.426;VQSLOD=3.92;culprit=FS GT:AD:DP:GQ:PL 1/1:0,46:48:99:1772,138,0
chr11   11797340        rs144215882     G       C       1716.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=46;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;QD=25.18;SOR=1.203;VQSLOD=4.42;culprit=FS GT:AD:DP:GQ:PL 1/1:0,45:46:99:1745,135,0
chr11   11798902        rs10831676      A       C       977.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=29;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=33.72;SOR=1.071;VQSLOD=7.07;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,29:29:86:1006,86,0
chr11   11799086        rs10765888      A       G       1257.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=34;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=32.90;SOR=1.085;VQSLOD=7.66;culprit=QD      GT:AD:DP:GQ:PL 1/1:0,34:34:99:1286,102,0
chr11   11801491        rs4910421       C       G       1030.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=30;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=27.93;SOR=1.255;VQSLOD=6.15;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,29:29:87:1059,87,0
chr11   11801735        rs369035976     G       C       411.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=-8.590e-01;DB;DP=33;ExcessHet=3.0103;FS=3.180;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;QD=13.28;ReadPosRankSum=-6.550e-01;SOR=0.293;VQSLOD=2.37;culprit=SOR GT:AD:DP:GQ:PL 0/1:17,14:31:99:440,0,519
chr11   11802003        rs7120452       C       T       1460.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=38;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=32.73;SOR=1.179;VQSLOD=7.04;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,38:38:99:1489,114,0
chr11   11802708        rs1380144972    AT      A       887.73 PASS    AC=2;AF=1.00;AN=2;DB;DP=25;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;QD=32.64;SOR=1.230;VQSLOD=5.14;culprit=FS GT:AD:DP:GQ:PGT:PID:PL:PS       1|1:0,21:21:63:1|1:11802708_AT_A:925,63,0:11802708
chr11   11802727        rs7123478       G       T       1054.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=25;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;QD=30.78;SOR=1.136;VQSLOD=4.74;culprit=FS GT:AD:DP:GQ:PGT:PID:PL:PS       1|1:0,25:25:75:1|1:11802708_AT_A:1083,75,0:11802708
chr12   11791178        rs2724624       T       A       1019.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=28;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=26.33;SOR=0.846;VQSLOD=6.35;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,26:26:78:1048,78,0
chr12   11791291        rs2856332       C       T       1291.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=34;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=36.54;SOR=0.811;VQSLOD=7.32;culprit=QD      GT:AD:DP:GQ:PL 1/1:0,34:34:99:1320,102,0
chr12   11791394        rs762718        A       G       421.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=-2.033e+00;DB;DP=31;ExcessHet=3.0103;FS=1.412;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=13.61;ReadPosRankSum=1.13;SOR=1.112;VQSLOD=3.99;culprit=ReadPosRankSum        GT:AD:DP:GQ:PL 0/1:16,15:31:99:450,0,555
chr12   11791542        rs2723824       G       C       1435.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=38;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=24.69;SOR=0.914;VQSLOD=5.14;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,38:38:99:1464,114,0
chr12   11791756        rs5796467       A       ATGT    1702.73 PASS    AC=2;AF=1.00;AN=2;DB;DP=43;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=28.71;SOR=1.236;VQSLOD=6.00;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,39:39:99:1740,117,0
chr12   11791772        rs2710283       T       G       1254.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=40;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=33.02;SOR=0.798;VQSLOD=6.70;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,38:38:99:1283,113,0
chr12   11791918        rs2724623       G       C       1814.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=51;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=32.31;SOR=0.909;VQSLOD=5.94;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,48:49:99:1843,144,0
chr12   11791976        rs2710282       T       C       1400.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=47;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=31.13;SOR=0.874;VQSLOD=5.75;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,45:46:99:1429,134,0
chr12   11792145        rs2710281       T       C       1031.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=40;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=29.48;SOR=0.804;VQSLOD=5.49;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,35:36:99:1060,104,0
chr12   11792326        rs2723823       T       C       1181.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=38;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=26.36;SOR=1.061;VQSLOD=6.73;culprit=QD      GT:AD:DP:GQ:PL 1/1:0,33:36:99:1210,99,0
chr12   11792686        rs5796468       C       CA      443.82 PASS    AC=2;AF=1.00;AN=2;BaseQRankSum=-1.424e+00;DB;DP=23;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=26.11;ReadPosRankSum=0.773;SOR=0.551;VQSLOD=3.46;culprit=FS     GT:AD:DP:GQ:PL 1/1:1,16:18:17:481,17,0
chr12   11793018        rs2724622       A       C       1218.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=39;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=32.94;SOR=0.859;VQSLOD=7.67;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,37:37:99:1247,110,0
chr12   11795015        rs2710280       T       G       1672.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=49;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=33.38;SOR=1.067;VQSLOD=6.84;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,46:48:99:1701,138,0
chr12   11796185        rs71981297      TGTGA   T       1762.73 PASS    AC=2;AF=1.00;AN=2;DB;DP=42;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=31.56;SOR=1.151;VQSLOD=7.24;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,40:40:99:1800,121,0
chr12   11796779        rs35668980      T       TG      835.73 PASS    AC=2;AF=1.00;AN=2;DB;DP=24;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=27.70;SOR=1.085;VQSLOD=5.90;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,21:22:65:873,65,0
chr12   11797291        rs60795005      TC      T       2115.73 PASS    AC=2;AF=1.00;AN=2;DB;DP=50;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=26.94;SOR=0.963;VQSLOD=5.64;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,48:48:99:2153,145,0
chr12   11798181        rs2724620       C       T       1185.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=33;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=28.35;SOR=0.756;VQSLOD=6.81;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,31:31:93:1214,93,0
chr12   11799490        rs762721        A       C       1099.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=36;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=31.42;SOR=1.148;VQSLOD=7.19;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,35:35:99:1128,105,0
chr12   11800969        rs2723840       C       G       1272.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=37;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=32.04;SOR=1.061;VQSLOD=7.00;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,36:36:99:1301,107,0
chr12   11801723        rs762722        C       G       1076.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=32;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=34.73;SOR=0.892;VQSLOD=7.39;culprit=QD      GT:AD:DP:GQ:PL 1/1:0,31:31:88:1105,88,0
chr16   11790688        rs8059377       C       A       649.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=3.57;DB;DP=39;ExcessHet=3.0103;FS=7.216;MLEAC=1;MLEAF=0.500;MQRankSum=-8.470e-01;POSITIVE_TRAIN_SITE;QD=17.10;ReadPosRankSum=0.059;SOR=1.397;VQSLOD=2.01;culprit=FS   GT:AD:DP:GQ:PL 0/1:17,21:38:99:678,0,361
chr16   11791587        rs72782733      C       A       260.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=3.10;DB;DP=27;ExcessHet=3.0103;FS=0.000;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=10.03;ReadPosRankSum=1.40;SOR=0.963;VQSLOD=4.63;culprit=FS GT:AD:DP:GQ:PL 0/1:17,9:26:99:289,0,516
chr16   11792735        rs6498245       A       G       1245.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=41;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=31.14;SOR=0.902;VQSLOD=7.58;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,40:40:99:1274,120,0
chr16   11793313        rs6498246       G       A       298.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=1.96;DB;DP=33;ExcessHet=3.0103;FS=0.000;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=9.96;ReadPosRankSum=-1.360e+00;SOR=0.693;VQSLOD=4.77;culprit=FS     GT:AD:DP:GQ:PL 0/1:19,11:31:99:327,0,478
chr16   11793532        rs550786318     C       CA      711.73 PASS    AC=2;AF=1.00;AN=2;DB;DP=24;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=33.89;SOR=3.816;VQSLOD=3.20;culprit=SOR     GT:AD:DP:GQ:PL 1/1:0,21:21:63:749,63,0
chr16   11793674        rs8056774       A       T       748.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=-9.360e-01;DB;DP=30;ExcessHet=3.0103;FS=1.664;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=24.96;ReadPosRankSum=-3.750e-01;SOR=0.610;VQSLOD=1.64;culprit=QD      GT:AD:DP:GQ:PL 0/1:8,22:30:99:777,0,231
chr16   11793773        rs62038760      A       G       733.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=-2.075e+00;DB;DP=39;ExcessHet=3.0103;FS=2.826;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=19.31;ReadPosRankSum=0.493;SOR=0.509;VQSLOD=3.60;culprit=FS   GT:AD:DP:GQ:PL 0/1:15,23:38:99:762,0,490
chr16   11793792        rs8061475       T       C       1466.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=40;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=33.53;SOR=0.793;VQSLOD=7.52;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,40:40:99:1495,120,0
chr16   11795063        rs8048397       C       T       383.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=1.31;DB;DP=23;ExcessHet=3.0103;FS=1.707;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=17.44;ReadPosRankSum=0.495;SOR=0.368;VQSLOD=3.09;culprit=FS GT:AD:DP:GQ:PL 0/1:10,12:22:99:412,0,311
chr16   11795203        rs8053548       T       C       212.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=-3.116e+00;DB;DP=20;ExcessHet=3.0103;FS=4.534;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=11.20;ReadPosRankSum=-4.540e-01;SOR=0.435;VQSLOD=1.59;culprit=QD      GT:AD:DP:GQ:PL 0/1:8,11:19:99:241,0,266
chr16   11795236        rs11864383      G       C       235.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=-4.720e-01;DB;DP=22;ExcessHet=3.0103;FS=4.123;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=11.23;ReadPosRankSum=-1.780e-01;SOR=1.288;VQSLOD=1.72;culprit=QD      GT:AD:DP:GQ:PL 0/1:12,9:21:99:264,0,408
chr16   11797059        rs755431804     C       CGG     121.73 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=1.07;DB;DP=7;ExcessHet=3.0103;FS=8.451;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;QD=17.39;ReadPosRankSum=-8.420e-01;SOR=1.402;VQSLOD=-8.775e-01;culprit=FS   GT:AD:DP:GQ:PL 0/1:3,4:7:99:159,0,114
chr16   11797264        rs148383532     G       A       227.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=2.24;DB;DP=10;ExcessHet=3.0103;FS=0.000;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;QD=22.78;ReadPosRankSum=0.755;SOR=0.527;VQSLOD=2.17;culprit=FS     GT:AD:DP:GQ:PL 0/1:3,7:10:61:256,0,61
chr16   11797505        rs28495625      C       A       596.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=1.38;DB;DP=31;ExcessHet=3.0103;FS=0.000;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=19.25;ReadPosRankSum=-4.100e-02;SOR=0.473;VQSLOD=4.16;culprit=FS    GT:AD:DP:GQ:PL 0/1:11,20:31:99:625,0,304
chr16   11798194        rs3960407       A       G       414.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=-2.010e-01;DB;DP=41;ExcessHet=3.0103;FS=4.831;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=10.37;ReadPosRankSum=0.224;SOR=1.545;VQSLOD=1.48;culprit=QD   GT:AD:DP:GQ:PL 0/1:25,15:40:99:443,0,827
chr16   11799021        rs566786090     C       CAAA    136.73 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=1.98;DB;DP=19;ExcessHet=3.0103;FS=2.077;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;QD=8.04;ReadPosRankSum=-1.690e+00;SOR=0.850;VQSLOD=0.540;culprit=QD        GT:AD:DP:GQ:PL 0/1:12,5:17:99:174,0,445
chr16   11799663        rs891210        C       G       335.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=1.22;DB;DP=31;ExcessHet=3.0103;FS=8.700;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=11.19;ReadPosRankSum=-2.750e-01;SOR=1.261;VQSLOD=2.21;culprit=FS    GT:AD:DP:GQ:PL 0/1:18,12:30:99:364,0,594
chr16   11800064        rs8055639       G       T       418.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=3.50;DB;DP=27;ExcessHet=3.0103;FS=1.583;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=16.11;ReadPosRankSum=0.777;SOR=0.346;VQSLOD=3.79;culprit=FS GT:AD:DP:GQ:PL 0/1:13,13:27:99:447,0,375
chr16   11800198        rs2160184       A       T       478.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=-3.700e-01;DB;DP=35;ExcessHet=3.0103;FS=7.319;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=14.08;ReadPosRankSum=0.639;SOR=1.541;VQSLOD=2.49;culprit=FS   GT:AD:DP:GQ:PL 0/1:18,16:34:99:507,0,615
chr16   11800358        rs2110620       T       C       1168.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=27;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=28.67;SOR=0.765;VQSLOD=5.86;culprit=FS      GT:AD:DP:GQ:PGT:PID:PL:PS 1|1:0,27:27:81:1|1:11800358_T_C:1197,81,0:11800358
chr16   11800360        rs2110619       T       C       1168.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=27;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=25.80;SOR=0.765;VQSLOD=6.15;culprit=FS      GT:AD:DP:GQ:PGT:PID:PL:PS 1|1:0,27:27:81:1|1:11800358_T_C:1197,81,0:11800358
chr16   11800859        rs56293020      T       C       1517.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=39;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;QD=30.20;SOR=1.236;VQSLOD=5.44;culprit=FS GT:AD:DP:GQ:PGT:PID:PL:PS       1|1:0,38:39:99:1|1:11800859_T_C:1546,114,0:11800859
chr16   11800860        rs76419258      A       G       479.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=-1.450e+00;DB;DP=39;ExcessHet=3.0103;FS=1.310;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;QD=12.97;ReadPosRankSum=1.56;SOR=1.051;VQSLOD=2.43;culprit=FS        GT:AD:DP:GQ:PGT:PID:PL:PS       0|1:17,20:38:99:0|1:11800859_T_C:508,0,482:11800859
chr16   11801296        rs6498247       G       A       406.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=1.84;DB;DP=22;ExcessHet=3.0103;FS=4.359;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=20.34;ReadPosRankSum=1.07;SOR=0.132;VQSLOD=2.35;culprit=SOR GT:AD:DP:GQ:PGT:PID:PL:PS       0|1:9,11:21:99:0|1:11801296_G_A:435,0,343:11801296
chr16   11801298        rs6498248       G       A       406.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=1.76;DB;DP=21;ExcessHet=3.0103;FS=4.359;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=20.34;ReadPosRankSum=1.07;SOR=0.132;VQSLOD=2.34;culprit=SOR GT:AD:DP:GQ:PGT:PID:PL:PS       0|1:9,11:21:99:0|1:11801296_G_A:435,0,343:11801296
chr16   11801523        rs6498249       T       G       807.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=23;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=35.82;SOR=0.776;VQSLOD=5.78;culprit=QD      GT:AD:DP:GQ:PL 1/1:0,22:23:66:836,66,0
chr16   11801693        rs3905565       C       G       180.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=0.095;DB;DP=29;ExcessHet=3.0103;FS=0.000;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=6.23;ReadPosRankSum=0.448;SOR=0.903;VQSLOD=4.52;culprit=QD GT:AD:DP:GQ:PL 0/1:20,9:29:99:209,0,618
chr16   11801707        rs7206424       C       G       209.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=-6.220e-01;DB;DP=32;ExcessHet=3.0103;FS=1.607;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=6.99;ReadPosRankSum=-7.250e-01;SOR=1.274;VQSLOD=1.60;culprit=QD       GT:AD:DP:GQ:PL 0/1:21,9:30:99:238,0,706
chr16   11801785        rs12446699      C       T       653.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=3.68;DB;DP=29;ExcessHet=3.0103;FS=1.617;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=23.35;ReadPosRankSum=-1.378e+00;SOR=0.905;VQSLOD=2.55;culprit=FS    GT:AD:DP:GQ:PL 0/1:9,19:28:99:682,0,196
chr16   11802159        rs4538034       G       A       482.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=1.15;DB;DP=34;ExcessHet=3.0103;FS=1.377;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=14.20;ReadPosRankSum=-3.450e-01;SOR=1.218;VQSLOD=3.65;culprit=ReadPosRankSum        GT:AD:DP:GQ:PL 0/1:17,17:34:99:511,0,471
chr16   11802382        rs35377237      T       TG      613.73 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=0.102;DB;DP=43;ExcessHet=3.0103;FS=1.261;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;QD=16.15;ReadPosRankSum=1.72;SOR=1.061;VQSLOD=1.06;culprit=ReadPosRankSum GT:AD:DP:GQ:PGT:PID:PL:PS       0|1:21,17:39:99:0|1:11802382_T_TG:651,0,831:11802382
chr16   11802387        rs35820990      G       T       661.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=2.87;DB;DP=43;ExcessHet=3.0103;FS=2.699;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;QD=16.54;ReadPosRankSum=1.69;SOR=1.179;VQSLOD=1.71;culprit=ReadPosRankSum GT:AD:DP:GQ:PGT:PID:PL:PS       0|1:22,18:41:99:0|1:11802382_T_TG:690,0,848:11802382
chr16   11802389        rs35349341      G       T       661.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=2.51;DB;DP=43;ExcessHet=3.0103;FS=2.699;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;QD=16.54;ReadPosRankSum=1.80;SOR=1.179;VQSLOD=1.67;culprit=ReadPosRankSum GT:AD:DP:GQ:PGT:PID:PL:PS       0|1:22,18:41:99:0|1:11802382_T_TG:690,0,848:11802382
chr16   11802625        rs7195390       T       C       425.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=-3.395e+00;DB;DP=32;ExcessHet=3.0103;FS=0.000;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=13.73;ReadPosRankSum=2.31;SOR=0.910;VQSLOD=4.45;culprit=FS    GT:AD:DP:GQ:PL 0/1:12,19:31:99:454,0,376
chr18   11790984        rs772652795     TCC     T       894.73 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=0.855;DB;DP=50;ExcessHet=3.0103;FS=0.000;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;QD=18.26;ReadPosRankSum=-6.280e-01;SOR=0.784;VQSLOD=2.56;culprit=FS       GT:AD:DP:GQ:PL 0/1:25,24:49:99:932,0,968
chr18   11791216        rs1863121       G       A       1248.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=33;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=24.81;SOR=0.693;VQSLOD=5.14;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,32:32:96:1277,96,0
chr18   11791733        rs9303747       C       G       1218.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=32;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=31.25;SOR=1.911;VQSLOD=5.55;culprit=FS      GT:AD:DP:GQ:PL 1/1:0,32:32:96:1247,96,0
chr18   11792239        rs72867020      A       T       224.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=0.485;DB;DP=24;ExcessHet=3.0103;FS=1.685;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=9.77;ReadPosRankSum=-4.480e-01;SOR=1.230;VQSLOD=2.89;culprit=FS    GT:AD:DP:GQ:PL 0/1:15,8:24:99:253,0,522
chr18   11793111        rs10401123      G       C       353.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=0.549;DB;DP=41;ExcessHet=3.0103;FS=4.720;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=9.07;ReadPosRankSum=1.51;SOR=0.754;VQSLOD=1.29;culprit=QD GT:AD:DP:GQ:PL 0/1:25,14:39:99:382,0,741
chr18   11793324        rs10401100      T       C       424.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=0.492;DB;DP=37;ExcessHet=3.0103;FS=1.365;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=12.49;ReadPosRankSum=-1.220e-01;SOR=0.603;VQSLOD=4.15;culprit=ReadPosRankSum       GT:AD:DP:GQ:PL 0/1:19,15:34:99:453,0,528
chr18   11794528        rs4797579       A       G       304.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=-3.668e+00;DB;DP=28;ExcessHet=3.0103;FS=6.239;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=12.19;ReadPosRankSum=0.00;SOR=1.492;VQSLOD=1.97;culprit=SOR   GT:AD:DP:GQ:PL 0/1:13,12:26:99:333,0,461
chr18   11795390        rs1339164174    CA      C       296.73 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=0.963;DB;DP=25;ExcessHet=3.0103;FS=7.402;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;QD=14.84;ReadPosRankSum=1.27;SOR=2.001;VQSLOD=-5.690e-02;culprit=FS       GT:AD:DP:GQ:PL 0/1:7,13:23:99:334,0,187
chr18   11796575        rs760136358     C       CAAA    61.73   PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=-7.810e-01;DB;DP=16;ExcessHet=3.0103;FS=0.000;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;QD=5.14;ReadPosRankSum=-9.780e-01;SOR=0.905;VQSLOD=2.58;culprit=QD   GT:AD:DP:GQ:PGT:PID:PL:PS       0|1:9,3:14:99:0|1:11796575_C_CAAA:99,0,363:11796575
chr18   11796596        rs8098035       C       A       102.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=2.98;DB;DP=17;ExcessHet=3.0103;FS=0.000;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;QD=7.34;ReadPosRankSum=0.429;SOR=0.693;VQSLOD=2.92;culprit=QD      GT:AD:DP:GQ:PGT:PID:PL:PS       0|1:10,4:16:99:0|1:11796575_C_CAAA:131,0,392:11796575
chr18   11797030        rs9944802       T       C       231.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=-1.940e+00;DB;DP=30;ExcessHet=3.0103;FS=8.923;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=8.28;ReadPosRankSum=-8.320e-01;SOR=1.706;VQSLOD=0.956;culprit=QD      GT:AD:DP:GQ:PL 0/1:18,10:29:99:260,0,589
chr18   11797740        rs143546710     CAGAG   C       530.73 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=-6.270e-01;DB;DP=31;ExcessHet=3.0103;FS=1.518;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=18.95;ReadPosRankSum=-7.800e-02;SOR=1.032;VQSLOD=3.33;culprit=FS      GT:AD:DP:GQ:PL 0/1:13,15:28:99:568,0,480
chr18   11799183        rs9962277       C       T       501.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=0.649;DB;DP=25;ExcessHet=3.0103;FS=1.760;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=20.91;ReadPosRankSum=-1.777e+00;SOR=0.697;VQSLOD=2.32;culprit=FS   GT:AD:DP:GQ:PL 0/1:8,16:24:99:530,0,224
chr18   11800067        rs10468681      C       A       280.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=1.61;DB;DP=22;ExcessHet=3.0103;FS=0.000;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=14.04;ReadPosRankSum=0.686;SOR=0.722;VQSLOD=5.54;culprit=FS GT:AD:DP:GQ:PL 0/1:11,9:20:99:309,0,381
chr18   11800408        rs9303748       C       T       682.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=0.861;DB;DP=42;ExcessHet=3.0103;FS=1.263;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=17.07;ReadPosRankSum=-6.600e-02;SOR=1.136;VQSLOD=4.16;culprit=FS   GT:AD:DP:GQ:PL 0/1:18,22:41:99:711,0,556
chr18   11800620        rs72867032      G       C       636.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=-9.440e-01;DB;DP=46;ExcessHet=3.0103;FS=0.000;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=14.15;ReadPosRankSum=-1.370e-01;SOR=0.807;VQSLOD=4.27;culprit=FS      GT:AD:DP:GQ:PL 0/1:24,21:45:99:665,0,786
chr18   11801730        rs34858453      GT      G       394.73 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=2.25;DB;DP=36;ExcessHet=3.0103;FS=3.174;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=10.96;ReadPosRankSum=0.185;SOR=1.445;VQSLOD=2.38;culprit=QD GT:AD:DP:GQ:PL 0/1:24,12:36:99:432,0,972
chr18   11802303        rs10048315      G       A       707.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=-8.490e-01;DB;DP=53;ExcessHet=3.0103;FS=2.488;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=15.06;ReadPosRankSum=0.383;SOR=0.856;VQSLOD=1.64;culprit=FS   GT:AD:DP:GQ:PL 0/1:24,23:47:99:736,0,799
chr18   11802505        rs10048316      C       T       676.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=0.347;DB;DP=52;ExcessHet=3.0103;FS=2.373;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=13.27;ReadPosRankSum=0.904;SOR=1.059;VQSLOD=2.46;culprit=FS        GT:AD:DP:GQ:PL 0/1:29,22:51:99:705,0,948
chr18   11802992        rs9303749       C       T       444.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=4.58;DB;DP=39;ExcessHet=3.0103;FS=7.194;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=11.40;ReadPosRankSum=-1.430e+00;SOR=1.361;VQSLOD=2.19;culprit=ReadPosRankSum        GT:AD:DP:GQ:PL 0/1:24,15:39:99:473,0,497
chr18   11803083        rs9972997       T       C       605.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=-1.501e+00;DB;DP=42;ExcessHet=3.0103;FS=2.694;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=14.42;ReadPosRankSum=1.03;SOR=0.825;VQSLOD=3.28;culprit=FS    GT:AD:DP:GQ:PL 0/1:21,21:42:99:634,0,667
chr19   11791513        rs12977679      A       G       590.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=-1.250e+00;DB;DP=40;ExcessHet=3.0103;FS=2.636;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=15.15;ReadPosRankSum=-1.016e+00;SOR=0.561;VQSLOD=2.60;culprit=FS      GT:AD:DP:GQ:PL 0/1:19,20:40:99:619,0,607
chr19   11792756        rs10401430      A       G       86.77   PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=1.19;DB;DP=15;ExcessHet=3.0103;FS=0.000;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=5.78;ReadPosRankSum=0.327;SOR=0.941;VQSLOD=3.61;culprit=QD GT:AD:DP:GQ:PL 0/1:11,4:15:99:115,0,310
chr19   11792786        rs12986106      C       A       80.77   PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=0.832;DB;DP=13;ExcessHet=3.0103;FS=0.000;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=6.21;ReadPosRankSum=-1.500e-01;SOR=1.329;VQSLOD=1.48;culprit=QD    GT:AD:DP:GQ:PL 0/1:9,4:13:99:109,0,273
chr19   11793268        rs371071500     CT      C       180.73 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=0.765;DB;DP=9;ExcessHet=3.0103;FS=0.000;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;QD=20.08;ReadPosRankSum=0.00;SOR=0.859;VQSLOD=2.68;culprit=FS      GT:AD:DP:GQ:PL 0/1:2,7:9:43:218,0,43
chr19   11794128        rs425995        A       G       495.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=-4.760e-01;DB;DP=34;ExcessHet=3.0103;FS=3.041;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=14.58;ReadPosRankSum=0.570;SOR=1.302;VQSLOD=3.55;culprit=ReadPosRankSum       GT:AD:DP:GQ:PL 0/1:18,16:34:99:524,0,627
chr19   11794274        rs286223        G       A       570.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=2.13;DB;DP=38;ExcessHet=3.0103;FS=1.273;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=15.43;ReadPosRankSum=0.532;SOR=0.446;VQSLOD=4.03;culprit=FS GT:AD:DP:GQ:PL 0/1:19,18:37:99:599,0,575
chr19   11794528        rs7253875       A       G       391.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=-4.189e+00;DB;DP=33;ExcessHet=3.0103;FS=0.000;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=11.87;ReadPosRankSum=0.667;SOR=0.622;VQSLOD=5.62;culprit=FS   GT:AD:DP:GQ:PL 0/1:17,16:33:99:420,0,577
chr19   11795669        rs286225        C       T       48.77   PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=0.126;DB;DP=6;ExcessHet=3.0103;FS=3.979;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=9.75;ReadPosRankSum=0.126;SOR=0.859;VQSLOD=-8.980e-02;culprit=DP    GT:AD:DP:GQ:PL 0/1:2,3:5:35:77,0,35
chr19   11796087        rs286226        T       G       150.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=-2.803e+00;DB;DP=16;ExcessHet=3.0103;FS=5.193;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=9.42;ReadPosRankSum=-1.540e-01;SOR=0.532;VQSLOD=1.09;culprit=QD       GT:AD:DP:GQ:PL 0/1:9,7:16:99:179,0,319
chr19   11796906        rs286228        C       T       432.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=4.07;DB;DP=23;ExcessHet=3.0103;FS=3.925;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=18.82;ReadPosRankSum=0.839;SOR=0.223;VQSLOD=2.67;culprit=QD GT:AD:DP:GQ:PL 0/1:10,13:23:99:461,0,209
chr19   11796977        rs286229        T       C       496.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=-1.865e+00;DB;DP=24;ExcessHet=3.0103;FS=0.000;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=20.70;ReadPosRankSum=-1.504e+00;SOR=0.741;VQSLOD=4.76;culprit=FS      GT:AD:DP:GQ:PL 0/1:8,16:24:99:525,0,247
chr19   11797055        rs286230        A       G       435.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=-1.882e+00;DB;DP=27;ExcessHet=3.0103;FS=3.565;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=16.14;ReadPosRankSum=-9.960e-01;SOR=0.223;VQSLOD=3.41;culprit=ReadPosRankSum GT:AD:DP:GQ:PL 0/1:13,14:27:99:464,0,433
chr19   11797089        rs286231        T       A       565.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=2.38;DB;DP=30;ExcessHet=3.0103;FS=8.869;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=19.51;ReadPosRankSum=-1.307e+00;SOR=0.718;VQSLOD=1.39;culprit=FS    GT:AD:DP:GQ:PL 0/1:12,17:29:99:594,0,348
chr19   11797582        rs286232        A       G       338.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=-7.100e-02;DB;DP=28;ExcessHet=3.0103;FS=0.000;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=12.10;ReadPosRankSum=-2.462e+00;SOR=0.760;VQSLOD=5.15;culprit=FS      GT:AD:DP:GQ:PL 0/1:16,12:28:99:367,0,530
chr19   11797955        rs286233        A       G       612.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=-2.349e+00;DB;DP=33;ExcessHet=3.0103;FS=1.523;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=19.77;ReadPosRankSum=-1.829e+00;SOR=0.738;VQSLOD=3.86;culprit=FS      GT:AD:DP:GQ:PL 0/1:9,22:31:99:641,0,264
chr19   11798192        rs5827137       TGCCTG T       454.73 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=2.53;DB;DP=38;ExcessHet=3.0103;FS=8.940;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=14.67;ReadPosRankSum=0.461;SOR=0.349;VQSLOD=2.35;culprit=FS GT:AD:DP:GQ:PL 0/1:18,13:31:99:492,0,716
chr19   11798757        rs286234        G       A       703.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=5.16;DB;DP=41;ExcessHet=3.0103;FS=15.322;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=17.17;ReadPosRankSum=0.365;SOR=1.317;VQSLOD=1.41;culprit=FS        GT:AD:DP:GQ:PL 0/1:20,21:41:99:732,0,535
chr19   11799463        rs184056        C       T       97.77   PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=-1.173e+00;DB;DP=14;ExcessHet=3.0103;FS=0.000;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=6.98;ReadPosRankSum=0.065;SOR=0.631;VQSLOD=3.89;culprit=QD    GT:AD:DP:GQ:PL 0/1:9,5:14:99:126,0,299
chr19   11799502        rs34472985      C       CTT     69.73   PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=0.854;DB;DP=8;ExcessHet=3.0103;FS=0.000;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;QD=8.72;ReadPosRankSum=0.439;SOR=0.307;VQSLOD=1.44;culprit=FS      GT:AD:DP:GQ:PL 0/1:5,3:8:99:107,0,188
chr19   11799664        rs286236        G       A       430.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=2.42;DB;DP=24;ExcessHet=3.0103;FS=3.963;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;QD=18.73;ReadPosRankSum=-3.110e-01;SOR=0.442;VQSLOD=0.150;culprit=QD       GT:AD:DP:GQ:PL 0/1:10,13:23:99:459,0,280
chr19   11799679        rs286237        C       T       355.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=-6.700e-02;DB;DP=22;ExcessHet=3.0103;FS=6.373;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=16.17;ReadPosRankSum=-2.640e-01;SOR=0.707;VQSLOD=1.37;culprit=ReadPosRankSum GT:AD:DP:GQ:PL 0/1:10,12:22:99:384,0,308
chr19   11800515        rs529515412     CTTTT   C,CTTTTT        857.73 PASS    AC=1,1;AF=0.500,0.500;AN=2;DB;DP=32;ExcessHet=3.0103;FS=0.000;MLEAC=1,1;MLEAF=0.500,0.500;QD=32.99;SOR=1.022;VQSLOD=5.63;culprit=FS        GT:AD:DP:GQ:PL     1/2:0,13,13:26:99:895,413,548,520,0,489
chr19   11800731        rs286238        A       T       675.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=0.475;DB;DP=41;ExcessHet=3.0103;FS=3.092;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=17.78;ReadPosRankSum=-7.420e-01;SOR=0.438;VQSLOD=1.98;culprit=ReadPosRankSum       GT:AD:DP:GQ:PL 0/1:16,22:38:99:704,0,491
chr19   11800812        rs7260645       T       C       551.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=-8.080e-01;DB;DP=35;ExcessHet=3.0103;FS=0.000;MLEAC=1;MLEAF=0.500;MQRankSum=-8.600e-01;POSITIVE_TRAIN_SITE;QD=15.76;ReadPosRankSum=-4.970e-01;SOR=0.542;VQSLOD=5.09;culprit=FS        GT:AD:DP:GQ:PL 0/1:16,19:35:99:580,0,491
chr19   11801436        rs74180187      C       A       419.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=1.19;DB;DP=23;ExcessHet=3.0103;FS=1.744;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=18.25;ReadPosRankSum=-4.990e-01;SOR=0.283;VQSLOD=2.94;culprit=FS    GT:AD:DP:GQ:PGT:PID:PL:PS       0|1:10,13:23:99:0|1:11801436_C_A:448,0,297:11801436
chr19   11801438        rs4804172       T       C       828.77 PASS    AC=2;AF=1.00;AN=2;DB;DP=24;ExcessHet=3.0103;FS=0.000;MLEAC=2;MLEAF=1.00;POSITIVE_TRAIN_SITE;QD=32.51;SOR=1.179;VQSLOD=5.16;culprit=FS      GT:AD:DP:GQ:PGT:PID:PL:PS 1|1:0,23:23:69:1|1:11801436_C_A:857,69,0:11801436
chr19   11801891        rs431393        A       G       349.77 PASS    AC=1;AF=0.500;AN=2;BaseQRankSum=-1.831e+00;DB;DP=37;ExcessHet=3.0103;FS=2.870;MLEAC=1;MLEAF=0.500;MQRankSum=0.00;POSITIVE_TRAIN_SITE;QD=9.72;ReadPosRankSum=-7.430e-01;SOR=0.574;VQSLOD=2.77;culprit=QD       GT:AD:DP:GQ:PL 0/1:22,14:37:99:378,0,745

agh for 1298 A>C (rs1801131) I need to grep "rs1801131" which returns no matches. So nebula's pre-provided vcf file does not work... nebula does provide BAM and FASTQ files but these are a bigger hassle and I may do other things before returning to this...

A look at "Longevity Olympics" winning routine: This 55-year-old single mom makes less than $100k per year and is reverse aging without spending millions.

Sun, 10 Dec 2023 14:21:20 +0000

https://fortune.com/well/article/reverse-aging-habits-daily-routine-single-mom-budget/amp/

Julie Gibson Clark is aging at 0.665 of a year for every chronological year she lives and ranks no.2 in a global online longevity game.

ALEXA MIKHAIL

December 09, 2023 5:00 AM EST

Clark’s results are more than impressive. In a global online longevity game called the Rejuvenation Olympics, ranking 4,000 people’s pace of aging averages across six months, Clark is in second place. She ranks higher than no.6 Bryan Johnson, the wealthy tech founder who spends $2 million per year on reverse aging, and no. 19 Peter Diamandis, whose venture fund has put $500 million into growing technologies, many of which are aimed at research and development for healthy aging and extending lifespan, according to his website.

Unlike the tech millionaires using extreme anti-aging protocols like spending up to $1,000 an hour to see a rejuvenation doctor, Clark’s secret is somewhat ordinary. After all, she makes less than six figures a year and cannot afford to spend her savings trying to live forever since she will need enough to sustain her into extreme old age. She spends $27 a month on a gym membership and $79 a month on a supplement subscription from NOVOS, the company whose trial she entered and worked with to submit her results to the longevity leaderboard.

“Eventually the wheels will fall off the bus, and I’m like, well, mine aren’t falling off anytime soon,” Clark previously told Fortune. “So I’m going to do everything I can to keep the bus in good order.”

So what’s her daily routine? Turns out, you can do it, too. “This stuff has to just kind of be like brushing your teeth,” Clark says.

A vegetable-rich diet

Clark typically consumes about 16 ounces of vegetables daily, snacking on carrots, radishes, and peppers during the workday. The majority, though, she gets through salads and soups. Eating a diverse array of whole foods, such as a range of vegetables, is associated with a strong gut, which can boost the immune system and keep the body healthy.

Clark also limits the amount of refined sugars and grains she eats, which contain fewer nutrients than complex carbohydrates.

Strength and cardio

When Clark heads to the gym, she does a mix of cardio and strength workouts each week: Two days of upper body workouts with weights, two days of lower body with weights, and one day of strength-training targeting her midsection. She also does about 20 to 30 minutes of cardio four times a week.

On the weekends, Clark hikes, kayaks, plays pickleball, or takes a long walk.

Especially as people age, incorporating strength training into the week can help combat age-related muscle loss. Finding an exercise you enjoy can help you stick with it.

A sauna and a cold shower

At least three times a week, Clark uses the sauna for 20 minutes before taking a cold shower.

Longevity experts swear by cold and hot immersion to stress the body. As longevity expert Dr. Mark Hyman previously told Fortune, “a stress that doesn’t kill you makes you stronger.”

Heat immersion like a sauna can activate longevity pathways, he says. “You’ll end up increasing heat shock proteins which clean up all damaged proteins and boost your immune system and increase your cardiovascular health,” Hyman says.

A cold shower, similar to the cold plunge, can do more than improve alertness. It can release adrenaline and keep the body resilient—a biological process known as hormesis, which can reduce inflammation.

It's Time To Science The Sh** Out Of DunedinPACE

Sun, 13 Oct 2024 11:30:54 +0000

Epigenetic Test #9: Finally, A Younger Horvath Age Than the Chronological

Tue, 21 Nov 2023 19:58:30 +0000

Metabolomics thread

Fri, 02 Jun 2023 18:06:55 +0000

Lower choline is associated with more efficient fiber structure (same with higher NAA levels) [at least in ONE study]. I mean it shows that ageotypes are unique to specific people (you need spatial metabolomics) otherwise an elevation in one metabolite could mean many different things [it could mean higher turnover *or* higher degradation *or* inefficient turnover]

https://www.rapamycin.news/t/analysis-of-the-metabolomic-state-informs-on-metabolite-profiles-associated-with-disease-risk/5142

DMTPA, vanillylmandelic acid, PFAS increase aging

L-serine decreases aging

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9796397/ (methionine-sulfoxide...)

also somalogic webinars

Amphetamine neurotoxicity (or the opposite)

Mon, 03 Jun 2024 03:19:50 +0000

https://onlinelibrary.wiley.com/doi/10.1111/acel.14087

Quote

Pairwise comparisons (with Sidak corrections) further revealed that cortical spine density was significantly reduced in untreated aged rats compared to the density in untreated young animals (p = 1.694e-6). Comparisons of the two AMPH-treated groups with the untreated groups revealed that the drug increased cortical spine density significantly in aged rats (p = 6.541e-10), resulting in a spine density in aged rats that was similar to the density in the young rats. In contrast, AMPH did not increase spine density in the young rats (Figure 5b).

FIGURE 5
Open in figure viewer PowerPoint

Chronic amphetamine treatment augments dendritic spine density and favors a morphological shift toward mature spines in aged Fischer 344 rats. (a) Representative example of Golgi staining of basal dendrites in layers II/III of the prelimbic cortex (PL) and in the hippocampal dentate gyrus (DG) of control and amphetamine (AMPH)-treated groups. (b, c) Spine density in layer II/III of the PL and in the DG of the hippocampus. Each individual point represents the mean of three randomized dendritic segments from each rat for density analyses (n = 5–6 rats per group). Each animal is represented by a different color. ****p < 0.0001. (d, e) Analysis of shifts in the relative abundance of long thin and mushroom spines (classified as described in Section 2) after AMPH treatment in layer II/III of the PL (d) and in the DG of the hippocampus (e) **p < 0.01 compared with the corresponding vehicle control groups and young versus aged.

Similarly, in the hippocampus (Figure 5c), a mixed model analysis revealed a significant main effect of age [F_(1,88.885) = 16.021, p = 1.30e-4], and a significant interaction between age and drug [F_(1,88.885) = 25.591, p = 2.25e-6]. As in the cortex, pairwise comparisons (with Sidak corrections) revealed that hippocampal spine density was significantly reduced in untreated aged rats compared to untreated young rats (p = 2.317e-8). Comparisons between the AMPH treatment groups with the untreated control groups revealed that AMPH increased spine density significantly in the aged rats (p = 1.359e-9) but not in the young rats.

Morphological analyses of spines in the prelimbic cortex (Figure 5d) revealed that AMPH treatment induces spine remodeling characterized by a decrease in long thin (immature/transient) spines and an increase in mushroom (stable mature) spines in aged rats. A three-way ANOVA (spine subtype × age × treatment) analyzing the percentage of spines within each experimental group indicated a significant main effect between spine subtype × age interaction [F_(1,38) = 8.839; p = 0.0051] and significant main effect between all three factors (subtype × age × drug) [F_(1,38) = 35.82; p < 0.0001]. Post hoc comparisons indicated that in comparison with young rats, aged rats displayed a significant decrease in mushroom spines (p = 0.0012) concomitant with an upregulation in long thin immature spines (p = 0.004). These findings are consistent with previous studies demonstrating structural reorganization occurring in normal aging (Dickstein et al., 2007). Importantly, chronic AMPH upregulated the number of mushroom spines in comparison with untreated aged controls (p = 0.0012), a phenomenon that was not observed in the young rats.

Similarly, in the hippocampus (Figure 5e), a three-way ANOVA analyzing spine distribution revealed a significant effect between spine subtype and age [F_(1,20) = 5.73; p = 0.0266] and a significant main effect between all three factors [spine subtype × drug × age; F_(1,16) = 17.19; p = 0.0008]. Similar to the findings in the prelimbic cortex, post hoc comparisons revealed that untreated aged animals displayed a decrease in mushroom spine density compared to young controls (p = 0.0084) that is rescued by AMPH treatment (p = 0.0012). Together, these data indicate that the unfavorable loss of dendritic mushroom spines associated with normal aging can be improved by AMPH treatment (Figure 5e and Figure S2).

Just curious, anyone have a plan, or preps for global pandemic?

Sun, 26 Jan 2020 01:20:10 +0000

Right now this China coronavirus doesn't seem all that threatening considering there are less than 100 confirmed deaths (2000 infections) and the regular seasonal flu kills 250,000 to 500,000 every single year... but I was just curious if anyone has made any special plans for a global pandemic type scenario, seeing as how this group seems to be all about staying alive 😉

World health organization is saying this thing is about as contagious as the Spanish Flu virus was. Just in the last 3 days, it went from 20 million quarantined to 40 million, now 56 million. So far it has about a 3% kill rate (so even if you get it, those odds are pretty good). There are a lot of disturbing videos coming out of China the last couple days, you can see them on twitter, people collapsing on streets, huge volumes of people at the hospital, caretakers collapsing, bodies in hospitals.

Not really sure how practical it would be to prepare for a global pandemic in general. I guess you could get a good ventilator/respirator, maybe stockpile a few months food or something. Anyone actually do that stuff? I do have a small survival corner of my basement, but probably not adequate, and it was mostly stocked about 15 to 20 years ago. You know just your basics - guns, ammo, bows, sealed Mormon food storage (I actually went to one of their prepper centers and picked/packed it myself, fun times, they are nuts but didn't care that I was an outsider, they were very friendly), canned food, water purifiers, fire starters, one good respirator, dust masks, nitrile gloves. I don't actually expect to ever use this stuff though. And the food would only last a couple weeks.

Kynurenine/Tryptophan Ratio: More Insight Into The De Novo NAD Synthesis Pathway

Sun, 21 May 2023 13:50:41 +0000

The walnuts thread (they're the weird nut)

Thu, 20 Apr 2023 18:21:49 +0000

One source says they're the healthiest nut

https://www.lifeextension.com/magazine/2022/4/walnuts-extend-your-life

But I just ate a bag of walnuts today and noticed the HUGE difference between unexpired nuts and expired nuts. The expired nuts do not taste terrible - you have to compare them side by side to see the difference and it is huge

They are heavy in PUFAs, which makes them unusually prone to oxidation (of all the fatty acids, PUFAs have the highest benefits *and* highest harms)

https://photos.app.goo.gl/G8KUvDyTLfWJPbpv6

Quote

WHERE TO STORE WALNUTS

When you bring walnuts (shelled or in-shell) home from the store, the best place to store them is in your refrigerator or freezer, depending on when you’re going to use them. If you’re going to use the walnuts right away, place them in your refrigerator. If you’ll be storing them for a month or longer, store them in your freezer.

HOW TO STORE WALNUTS

If you buy walnuts in sealed packaging, you can store the walnuts in their original packaging in the refrigerator or freezer. Once you open the bag, transfer the walnuts to an airtight container to maintain freshness and then place them back in the refrigerator or freezer. If you buy bulk walnuts, either in-shell or shelled, place the walnuts in an airtight container for long-term cold storage.

Quote

Several nuts are among the dietary plants with the highest content of total antioxidants. Of the tree nuts, walnuts, pecans and chestnuts have the highest contents of antioxidants. Walnuts contain more than 20 mmol antioxidants per 100 g, mostly in the walnut pellicles. Peanuts (a legume) also contribute significantly to dietary intake of antioxidants. These data are in accordance with our present extended analysis of an earlier report on nut intake and death attributed to various diseases in the Iowa Women's Health Study. We observed that the hazard ratio for total death rates showed a U-shaped association with nut/peanut butter consumption. Hazard ratio was 0.89 (CI = 0.81-0.97) and 0.81 (CI = 0.75-0.88) for nut/peanut butter intake once per week and 1-4 times per week, respectively

The quality between walnuts seems to vary A LOT, it's crazy...

How concerned are you with PFAS/ways to reduce/blood testing?

Sun, 02 Jun 2024 03:01:10 +0000

PFAS is often in tea bags (this is why I remove the bag before pouring)...

Tejava uses giant tea bags, I might have to externally test it b/c I'm starting to drink a lot of Tejava (testing water is $70).

I'm not as concerned about PFAS as I am with microplastics, and I don't do much takeout, but amounts in tea could be concerning.

Astaxanthin (the best evidence that SOME antioxidants can still be pro-longevity)

Thu, 10 Aug 2023 20:19:46 +0000

Quote

constant DNA damage is a fact, and at least knowledge is empowering; thus, we can start doing something about it. Anyway, in 2010, a study looked at healthy 21.5 year old women (on average) that took placebo, 2 mg or 8 mg/day of astaxanthin for 8 weeks. Remarkably, both doses of astaxanthin significantly lowered the rate of DNA breakdown.5 One of the interesting take home messages here is that even people in their twenties are experiencing measurable DNA damage. They are already aging, they just don’t know it.

Seeking more evidence of astaxanthin’s power to combat free radicals, eyeball tissues in diabetic rats were examined for evidence of hyperglycemic induced, oxidative stress damage and its reversibility. Not only do high glucose levels increase the production of free oxygen radicals, it causes the depletion of cellular antioxidant defense capacities. Not unexpectedly, significant damage to the retina and surrounding eye tissues was noted in the control group. By comparison, the addition of astaxanthin to their diet, at a variety of concentrations, was extremely beneficial.

Quote

astaxanthin molecule reaches an individual cell, and we’ll get to transport later, it is able to incorporate into both the cell membrane and the mitochondrial membrane. The molecule inserts itself directly into the lipid bilayers, and spans the entire membrane.

Called a transmembrane orientation, this prime location allows the molecule to perform its magic in several subcellular components. As a direct result, astaxanthin is a far more potent free radical scavenger (ROS) than its molecular cousins. It is 200 times that of the other polyphenols, 150x anthocyanin, 75x alpha-lipoic acid, 550x greater than vitamin E, 54x greater than B-Carotene, 6000x greater than Vitamin C, and 800x that of Coenzyme Q.3

Running A Sub-4 Minute Mile: What's The Average Life Expectancy Increase?

Wed, 29 May 2024 10:58:15 +0000

General Health and Longevity Latest Topics

What is your grip strength?

Fasting (Without Calorie Restriction) And Calorie Restriction With Fasting Extend Lifespan

Drinking/tap water thread (and ideal water filters/pitchers)

Is carnosine worth it?

How Not To Age: Dr. Michael Greger

Healthy Tea Recommendations?

195,500 particles of microplastic **per gram** of apple? 126,150 particles **per gram** of broccoli?

Metformin - yet another u-turn?

Is C15 A Good Measure Of Aging?

[IMPORTANT] Favorite low-effort, lower-calorie food items that you can easily get from a store/use as fast food. #DiningGuide

Watercress Is The Healthiest Vegetable? Carrot is #26? Here’s A Chart To... 11

Garden Vegetable Soup 1 (trader joe's)

Aubrey de Grey resurfaces, predicts 50% chance of longevity escape velocity within 15 years

Red Light Therapy Improves Mitochondria Function, Eye Health, And Lifespan: Glen Jeffery, PhD

QEEG report of brainwaves with raw uV values (got them from someone who fed my data into neuroguide)

What are the most important genome/SNP/SNPedia sites to query from a VCF file of one's genome? How do I visualize my genomic data

A look at "Longevity Olympics" winning routine: This 55-year-old single mom makes less than $100k per year and is reverse aging without spending millions.

Julie Gibson Clark is aging at 0.665 of a year for every chronological year she lives and ranks no.2 in a global online longevity game.

A vegetable-rich diet

Strength and cardio

A sauna and a cold shower

It's Time To Science The Sh** Out Of DunedinPACE

Epigenetic Test #9: Finally, A Younger Horvath Age Than the Chronological

Metabolomics thread

Amphetamine neurotoxicity (or the opposite)

Just curious, anyone have a plan, or preps for global pandemic?

Kynurenine/Tryptophan Ratio: More Insight Into The De Novo NAD Synthesis Pathway

The walnuts thread (they're the weird nut)

WHERE TO STORE WALNUTS

HOW TO STORE WALNUTS

How concerned are you with PFAS/ways to reduce/blood testing?

Astaxanthin (the best evidence that **SOME** antioxidants can still be pro-longevity)

Running A Sub-4 Minute Mile: What's The Average Life Expectancy Increase?

195,500 particles of microplastic per gram of apple? 126,150 particles per gram of broccoli?

Astaxanthin (the best evidence that SOME antioxidants can still be pro-longevity)